Human Chr18 transcriptome dataset combined from the Illumina HiSeq, ONT MinION, and qPCR data
نویسندگان
چکیده
منابع مشابه
Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches
This study aimed to assess the feasibility of using the Oxford Nanopore Technologies (ONT) MinION long-read sequencer in reconstructing fully closed plasmid sequences from eight Enterobacteriaceae isolates of six different species with plasmid populations of varying complexity. Species represented were Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter cloacae, Serratia...
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BACKGROUND Dastarcus helophoroides is known as the most valuable natural enemy insect against many large-body longhorned beetles. The molecular mechanism of its long lifespan and reproduction makes it a unique resource for genomic research. However, molecular biological studies on this parasitic beetle are scarce, and genomic information for D. helophoroides is not currently available. Thus, tr...
متن کاملA single chromosome assembly of Bacteroides fragilis strain BE1 from Illumina and MinION nanopore sequencing data
BACKGROUND Second and third generation sequencing technologies have revolutionised bacterial genomics. Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads. RESULTS We sequen...
متن کاملBenchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix were sequenced. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts with known concentration. cDNA libraries were generated using a template switching protocol to facilitate the direct comparison betwee...
متن کاملCorrigendum: Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches
Received 27 July 2017; Accepted 27 July 2017 Author affiliations: 1 Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK; 2 Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA; 3 The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK; 4 Addenbrookes Ho...
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ژورنال
عنوان ژورنال: Data in Brief
سال: 2021
ISSN: 2352-3409
DOI: 10.1016/j.dib.2021.107130